Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.

Establishment of a Novel Human Megakaryoblastic Leukemia Cell Line , MEG - Ol , With Positive Philadelphia Chromosome

A megakaryoblastic cell line. designated MEG-Ol . was established from the bone marrow of a patient with blast crisis of Philadelphia (Ph1 ) chromosome-positive chronic myelogenous leukemia. MEG-Ol cells grew in single-cell suspension with a doubling time of 36 to 48 hours. Under the usual culture conditions. approximately half of the cells adhered to the culture flask with extention of pseudop...

Establishment of a human megakaryoblastic cell line (T-33) from chronic myelogenous leukemia in megakaryoblastic crisis.

A megakaryoblastic cell line, termed T-33, was established from the peripheral blood of a patient with Philadelphia chromosome-positive chronic myelogenous leukemia in megakaryoblastic crisis. T-33 cells have been maintained in RPMI 1640 medium containing 10% fetal calf serum in a single cell suspension with a doubling time of 24-36 h for over 2 years. Giemsa-banded karyotypes were female hyper...

Globin genes are actively transcribed in the human megakaryoblastic leukemia cell line MEG-01.

Expression of the Gb3/CD77 Synthase Gene in Megakaryoblastic Leukemia Cells

Expression levels of Gb3/CD77 synthase together with Gb3/CD77 antigen were analyzed using human hematopoietic tumor cell lines and normal cells. Among about 40 kinds of cells, Burkitt lymphoma cells showed the highest gene expression concomitant with the expression levels of Gb3/CD77. Unexpectedly, megakaryoblastic leukemia lines also expressed fairly high levels of mRNA of Gb3/CD77 synthase an...

Synergistic effect of imatinib mesylate and fludarabine combination on Philadelphia chromosome-positive chronic myeloid leukemia cell lines.

Fludarabine-containing combinations have additive cell killing against leukemic blasts in vitro. It has also been shown that imatinib mesylate combined with fludarabine or cladribine had an additive effect on CML CFU-GM cells. In this regard, we aimed to investigate the effect of fludarabine-imatinib mesylate combination against CML blastic phase cell lines K562 and Meg-01. XTT test was perform...